I did it as a test of expired glucagon, and was also doing mini-doses of it. It was 3 years past expiration.
On the first day, I went from 51 to 109, and that was with just 25 units.
Then I tried it a few days later and it still worked. I actually got 5 days out of it before it stopped working, not 3 days. I forgot a little about the details.
Here are the details: