Functional Insulin-Producing Cells Grown In Lab


#1

Mimicking Natural Development Yields Stem Cell Breakthrough, Opens Door to Type 1 Diabetes Cure

"UC San Francisco researchers have for the first time transformed human stem cells into mature insulin-producing cells, a major breakthrough in the effort to develop a cure for type 1 (T1) diabetes.

Replacing these cells, which are lost in patients with T1 diabetes, has long been a dream of regenerative medicine, but until now scientists had not been able to figure out how to produce cells in a lab dish that work as they do in healthy adults.

“We can now generate insulin-producing cells that look and act a lot like the pancreatic beta cells you and I have in our bodies. This is a critical step towards our goal of creating cells that could be transplanted into patients with diabetes,”


#2

I do believe this has been achieved with the use of spleen cells. IIRC it was found by accident when transplantimg spleen cells into the pancreas for some other reason, again I think it was foumd that where ever spleen cells are transplanted, they will replicate or change into the same type of cells in the area they are transplanted. I think it was first found in mice and then humans.


#3

This article just came out on 2-1-19, they are using stem cells to grow the beta cells which until now they couldn’t get to grow into adult functioning beta cells. It actually is a real breakthrough. I had heard of this a long time ago but I haven’t kept up on it I’ve pasted more of the article below, it’s worth a read…

"…Transplants of just the pancreatic “islets” – clusters of cells containing healthy beta cells – are currently in clinical trials, but still rely on pancreases from deceased donors.

That’s why Hebrok and other diabetes researchers have long hoped to use stem cells to grow healthy beta cells in the lab that could be transplanted into patients without the need to wait for a pancreas or islet transplant. But for years, scientists have been unable to figure out how to program stem cells into fully mature beta cells.

“The cells we and others were producing were getting stuck at an immature stage where they weren’t able to respond adequately to blood glucose and secrete insulin properly. It has been a major bottleneck for the field,” Hebrok said.

In the new study, Hebrok and colleagues, led by postdoctoral fellow Gopika Nair, PhD, realized that the key to getting lab-grown beta cells to fully mature lay in an overlooked facet of beta cell development – the physical process by which the cells separate from the rest of the pancreas and form the so-called islets of Langerhans.

“A key principle in biology is that form follows function, so we reasoned that the formation of islets might be an important process for beta cells to mature properly,” Nair said.

When the researchers replicated that process in lab dishes by artificially separating partially differentiated pancreatic stem cells and reforming them into islet-like clusters, the cells’ development suddenly leapt forward. Not only did the beta cells begin responding to blood sugar more like mature insulin-producing cells, but the entire islet “neighborhood” – including less well-understood alpha and delta cells – also seemed to develop in ways that had never been seen in a laboratory setting.

The researchers then transplanted these lab-grown “islets” into healthy mice and found that they were functional in a matter of days – producing insulin in response to blood sugar much like the animals’ own islets.

In collaboration with bioengineers, geneticists, and other colleagues at UCSF, Hebrok’s team is already working to move regenerative therapies from dream to reality, for instance by using CRISPR gene editing to make these cells transplantable into patients without the need for immune-suppressing drugs, or by screening drugs that could restore proper islet function in patients with T1 diabetes by protecting and expanding their few remaining beta cells to reboot pancreatic insulin production.

“Current therapeutics like insulin injections only treat the symptoms of the disease,” Nair said. “Our work points to several exciting avenues to finally finding a cure.”

“We’re finally able to move forward on a number of different fronts that were previously closed to us,” Hebrok added. “The possibilities seem endless.”


#4

It is interesting, and the reason I mentioned the spleen cell was that it was very similar but didnt need a transplant from an external donor, it was through using spleen cells from the same individual.